Difference between revisions of "Part:BBa Q04401"

(→‎Reuse)
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===Reuse===
 
===Reuse===
 
*This inverter was successfully reused by the [http://openwetware.org/wiki/IGEM:MIT/2006 2006 MIT iGEM team] to invert the output of a stationary phase promoter. (what BB #?)
 
*This inverter was successfully reused by the [http://openwetware.org/wiki/IGEM:MIT/2006 2006 MIT iGEM team] to invert the output of a stationary phase promoter. (what BB #?)
−
*'''[[User:Jasonk|Jasonk]] 02:44, 23 February 2008 (EST):'''In general, I think it's more likely to work when used on a high-copy (1 series) plasmid.  I've tried it on a 3 series, using <bbpart>F2621</bbpart>as the induction system and did not see flipping of the inverter.
+
*'''[[User:Jasonk|Jasonk]] 02:44, 23 February 2008 (EST):'''In general, I think it's more likely to work when used on a high-copy (1 series) plasmid.  I've tried it on a 3 series, using <bbpart>BBa_F2621</bbpart>as the induction system and did not see flipping of the inverter.

Revision as of 07:53, 23 February 2008

TetR-derived transcriptional inverter

This is a modified version of the tetR inverter Q04400 with a single base switch in the RBS: AAAGAGGAGAAA to AAAGAGGGGAAA.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Transfer curve for Q04401 measured in the Screening Plasmid(pSB1A10). Units are arbitrary. The image in top right is a dot plot based on concatenated flow cytometry data from 6 different arabinose induction levels (see pSB1A10for details). Main figure is the same dot plot data averaged to make the transfer curve more clear.



Reuse

  • This inverter was successfully reused by the [http://openwetware.org/wiki/IGEM:MIT/2006 2006 MIT iGEM team] to invert the output of a stationary phase promoter. (what BB #?)
  • Jasonk 02:44, 23 February 2008 (EST):In general, I think it's more likely to work when used on a high-copy (1 series) plasmid. I've tried it on a 3 series, using BBa_F2621as the induction system and did not see flipping of the inverter.