Difference between revisions of "Part:BBa Q04401"
(→Reuse) |
|||
| Line 22: | Line 22: | ||
===Reuse=== | ===Reuse=== | ||
| − | *This inverter was successfully reused by the [http://openwetware.org/wiki/IGEM:MIT/2006 2006 MIT iGEM team]. | + | *This inverter was successfully reused by the [http://openwetware.org/wiki/IGEM:MIT/2006 2006 MIT iGEM team] to invert the output of a stationary phase promoter. (what BB #?) |
*'''[[User:Jasonk|Jasonk]] 02:44, 23 February 2008 (EST):'''In general, I think it's more likely to work when used on a high-copy (1 series) plasmid. I've tried it on a 3 series, using <bbpart>F2621</bbpart> as the induction system and did not see flipping of the inverter. | *'''[[User:Jasonk|Jasonk]] 02:44, 23 February 2008 (EST):'''In general, I think it's more likely to work when used on a high-copy (1 series) plasmid. I've tried it on a 3 series, using <bbpart>F2621</bbpart> as the induction system and did not see flipping of the inverter. | ||
Revision as of 07:48, 23 February 2008
TetR-derived transcriptional inverter
This is a modified version of the tetR inverter Q04400 with a single base switch in the RBS: AAAGAGGAGAAA to AAAGAGGGGAAA.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Transfer curve for Q04401 measured in the Screening Plasmid. Units are arbitrary. (pSB1A10)
Reuse
- This inverter was successfully reused by the [http://openwetware.org/wiki/IGEM:MIT/2006 2006 MIT iGEM team] to invert the output of a stationary phase promoter. (what BB #?)
- Jasonk 02:44, 23 February 2008 (EST):In general, I think it's more likely to work when used on a high-copy (1 series) plasmid. I've tried it on a 3 series, using F2621 as the induction system and did not see flipping of the inverter.