Difference between revisions of "Part:BBa K2549010"

Revision as of 14:40, 6 October 2018

split intein Cfa C

This part is the C-terminal fragment of Cfa[1]. Cfa is a consensus sequence from an alignment of 73 naturally occurring DnaE inteins that are predicted to have fast splicing rates. Cfa demonstrates both rapid protein splicing and unprecedented thermal and chaotropic durability. The 122-124 residues of Cfa is mutated from EKD to GEP, which has been proved to imbue ultrafast DnaE split inteins with minimal extein dependence, thus improving split Intein-mediated protein cyclization[2]. Cfa’C is used in our amplifier to accomplish some complex logic functions. It can also be used by other iGEM teams to assembly their intein-based protein libraries.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 91
Illegal SapI.rc site found at 21

References

[1]Design of a Split Intein with Exceptional Protein Splicing Activity. Stevens AJ, Brown ZZ, Shah NH, ..., Cowburn D, Muir TW. J Am Chem Soc, 2016 Feb;138(7):2162-5 PMID: 26854538; DOI: 10.1021/jacs.5b13528

[2]A promiscuous split intein with expanded protein engineering applications. Stevens AJ, Sekar G, Shah NH, ..., Cowburn D, Muir TW. Proc Natl Acad Sci U S A, 2017 Aug;114(32):8538-8543 PMID: 28739907; DOI: 10.1073/pnas.1701083114

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 <partinfo>BBa_K2549010 short</partinfo>
-This part is the C-terminal fragment of Cfa. Cfa is a consensus sequence from an alignment of 73 naturally occurring DnaE inteins that are predicted to have fast splicing rates. Cfa demonstrates both rapid protein splicing and unprecedented thermal and chaotropic durability. The 122-124 residues of Cfa is mutated from EKD to GEP, which has been proved to imbue ultrafast DnaE split inteins with minimal extein dependence, thus improving split Intein-mediated protein cyclization. Cfa’C is used in our amplifier to accomplish some complex logic functions. It can also be used by other iGEM teams to assembly their intein-based protein libraries.
+This part is the C-terminal fragment of Cfa[1]. Cfa is a consensus sequence from an alignment of 73 naturally occurring DnaE inteins that are predicted to have fast splicing rates. Cfa demonstrates both rapid protein splicing and unprecedented thermal and chaotropic durability. The 122-124 residues of Cfa is mutated from EKD to GEP, which has been proved to imbue ultrafast DnaE split inteins with minimal extein dependence, thus improving split Intein-mediated protein cyclization[2]. Cfa’C is used in our amplifier to accomplish some complex logic functions. It can also be used by other iGEM teams to assembly their intein-based protein libraries.
 <!-- Add more about the biology of this part here