Difference between revisions of "Part:BBa K2886007"

 
Line 4: Line 4:
  
 
VEGF (vascular endothelial growth factor) is common ligand used in synthetic biology. And there are a lot of types of VEGF like VEGFA-121, VEGFA-165 and so on. VEGFA-121 was firstly introduced to iGEM by  iGEM12_Slovenia in 2012, which was from mouse. In 2017, iGEM17_SYSU-CHINA improved this part by isolating it from the initial composite part and submitting the counterpart in human.making VEGFA-121 more mobile and suitable for human cell lines.
 
VEGF (vascular endothelial growth factor) is common ligand used in synthetic biology. And there are a lot of types of VEGF like VEGFA-121, VEGFA-165 and so on. VEGFA-121 was firstly introduced to iGEM by  iGEM12_Slovenia in 2012, which was from mouse. In 2017, iGEM17_SYSU-CHINA improved this part by isolating it from the initial composite part and submitting the counterpart in human.making VEGFA-121 more mobile and suitable for human cell lines.
 +
 
This year, Fudan-CHINA makes new improvements to VEGFA to make it more useful. In Human there are two main types of VEGFA: VEGF-121 and VEGFA-165. Former teams introduced VEGFA-121 to iGEM and we this time we introduced another type VEGFA-165 to iGEM to enrich the chioces of iGEMers. According to literatures, VEGFA-165 has a higher affinity to heparin and might has higher activity than VEGFA-121. And VEGFA-165 is much more adopted by researcher in research.And what's more, Buying VEGFA-165 from companies might be very expensive, so we add a His tag that could help us to express and purify VEGFA-165 easily.And We did succeed in collecting purified VEGFA-165 with the part we constructed. What is also worth mentioning is that we could also us GS tag, but given it's even longer than VEGF itself, we turned to His tag ultimately.
 
This year, Fudan-CHINA makes new improvements to VEGFA to make it more useful. In Human there are two main types of VEGFA: VEGF-121 and VEGFA-165. Former teams introduced VEGFA-121 to iGEM and we this time we introduced another type VEGFA-165 to iGEM to enrich the chioces of iGEMers. According to literatures, VEGFA-165 has a higher affinity to heparin and might has higher activity than VEGFA-121. And VEGFA-165 is much more adopted by researcher in research.And what's more, Buying VEGFA-165 from companies might be very expensive, so we add a His tag that could help us to express and purify VEGFA-165 easily.And We did succeed in collecting purified VEGFA-165 with the part we constructed. What is also worth mentioning is that we could also us GS tag, but given it's even longer than VEGF itself, we turned to His tag ultimately.
 +
 
All in all, through our work, VEGFA becomes much more available and easy to use.
 
All in all, through our work, VEGFA becomes much more available and easy to use.
  

Revision as of 13:25, 6 October 2018


VEGF-His9

VEGF (vascular endothelial growth factor) is common ligand used in synthetic biology. And there are a lot of types of VEGF like VEGFA-121, VEGFA-165 and so on. VEGFA-121 was firstly introduced to iGEM by iGEM12_Slovenia in 2012, which was from mouse. In 2017, iGEM17_SYSU-CHINA improved this part by isolating it from the initial composite part and submitting the counterpart in human.making VEGFA-121 more mobile and suitable for human cell lines.

This year, Fudan-CHINA makes new improvements to VEGFA to make it more useful. In Human there are two main types of VEGFA: VEGF-121 and VEGFA-165. Former teams introduced VEGFA-121 to iGEM and we this time we introduced another type VEGFA-165 to iGEM to enrich the chioces of iGEMers. According to literatures, VEGFA-165 has a higher affinity to heparin and might has higher activity than VEGFA-121. And VEGFA-165 is much more adopted by researcher in research.And what's more, Buying VEGFA-165 from companies might be very expensive, so we add a His tag that could help us to express and purify VEGFA-165 easily.And We did succeed in collecting purified VEGFA-165 with the part we constructed. What is also worth mentioning is that we could also us GS tag, but given it's even longer than VEGF itself, we turned to His tag ultimately.

All in all, through our work, VEGFA becomes much more available and easy to use.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 166