Difference between revisions of "Part:BBa K2549004"

Revision as of 11:31, 6 October 2018

anti-GFP (LaG17)

Anti-GFP(LaG17) is a readily expressible recombinant nanobody which has a relatively low affinity and high specificity against GFP[1]. It can be used as the extracellular domain of the SynNotch, thus accomplishing the contact-dependent signal input against GFP.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 34

References

[1]A robust pipeline for rapid production of versatile nanobody repertoires. Fridy PC, Li Y, Keegan S, ..., Chait BT, Rout MP. Nat Methods, 2014 Dec;11(12):1253-60 PMID: 25362362; DOI: 10.1038/nmeth.3170

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2549004 short</partinfo>
-Anti-GFP(LaG17) is a readily expressible recombinant nanobody which has a relatively low affinity and high specificity against GFP. It can be used as the extracellular domain of the SynNotch, thus accomplishing the contact-dependent signal input against GFP.
+Anti-GFP(LaG17) is a readily expressible recombinant nanobody which has a relatively low affinity and high specificity against GFP[1]. It can be used as the extracellular domain of the SynNotch, thus accomplishing the contact-dependent signal input against GFP.
 <!-- Add more about the biology of this part here
@@ Line 12: / Line 12: @@
 <partinfo>BBa_K2549004 SequenceAndFeatures</partinfo>
+===References===
+[1]A robust pipeline for rapid production of versatile nanobody repertoires. Fridy PC, Li Y, Keegan S, ..., Chait BT, Rout MP. Nat Methods, 2014 Dec;11(12):1253-60 PMID: 25362362; DOI: 10.1038/nmeth.3170
 <!-- Uncomment this to enable Functional Parameter display