Difference between revisions of "Part:BBa K2842680"
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[[File:T--UCL--IP RFP GFP gel.png|400px|thumb|left|
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[[File:T--UCL--bsaI digests IP RFP GFP.png|400px|thumb|left|
 
<center>'''Figure 1: BsaI digestions'''</center>
 
<center>'''Figure 1: BsaI digestions'''</center>
  

Revision as of 11:02, 6 October 2018


Intein Monomer 1: RFP reporter flanked with orthogonal inteins


Figure 1: BsaI digestions

(1) BsaI digested Intein Passenger BBa_K2842669
(2) BsaI digested RFP inteins BBa_K2842680
(3) BsaI digested GFP inteins BBa_K2842690





















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Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1165
    Illegal BsaI.rc site found at 28
    Illegal SapI site found at 903
    Illegal SapI.rc site found at 213