Difference between revisions of "Part:BBa K2842669"
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(1) BsaI digested Intein Passenger [https://parts.igem.org/Part:BBa_K2842669 BBa_K2842669] <br />
 
(1) BsaI digested Intein Passenger [https://parts.igem.org/Part:BBa_K2842669 BBa_K2842669] <br />
 
(2) BsaI digested RFP inteins [https://parts.igem.org/Part:BBa_K2842680 BBa_K2842680] <br />
 
(2) BsaI digested RFP inteins [https://parts.igem.org/Part:BBa_K2842680 BBa_K2842680] <br />
−
(3) BsaI digested GFP inteins [https://parts.igem.org/Part:BBa_K2842690 BBa_K2842690]
+
(3) BsaI digested GFP inteins [https://parts.igem.org/Part:BBa_K2842690 BBa_K2842690] <br />
 +
*edited to show the relevant bands next to each other
 
</p>''' ]]
 
</p>''' ]]
  

Revision as of 11:00, 6 October 2018


mScarlet reporter with TerL-C intein on the N terminus

Figure 1: BsaI digestions

(1) BsaI digested Intein Passenger BBa_K2842669
(2) BsaI digested RFP inteins BBa_K2842680
(3) BsaI digested GFP inteins BBa_K2842690
*edited to show the relevant bands next to each other

-

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 941
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1224
    Illegal BsaI.rc site found at 28
    Illegal SapI site found at 1130
    Illegal SapI.rc site found at 419