Difference between revisions of "Part:BBa K2549003"

 
Line 3: Line 3:
 
<partinfo>BBa_K2549003 short</partinfo>
 
<partinfo>BBa_K2549003 short</partinfo>
  
−
Anti-GFP(LaG16) is a readily expressible recombinant nanobody which has a high affinity and high specificity against GFP. It can be used as the extracellular domain of the SynNotch, thus accomplishing the contact-dependent signal input against GFP. When fused with LaG2 using a flexible glycine-rich peptide linker to form a dimerization, ultra-high affinity can be conducted.
+
Anti-GFP(LaG16) is a readily expressible recombinant nanobody which has a high affinity and high specificity against GFP. It can be used as the extracellular domain of the SynNotch, thus accomplishing the contact-dependent signal input against GFP. When fused with LaG2([[Part:BBa_K2549002|Click here to see!]]) using a flexible glycine-rich peptide linker to form a dimerization, ultra-high affinity can be conducted[1].
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
Line 12: Line 12:
 
<partinfo>BBa_K2549003 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2549003 SequenceAndFeatures</partinfo>
  
 +
===References===
 +
[1]A robust pipeline for rapid production of versatile nanobody repertoires. Fridy PC, Li Y, Keegan S, ..., Chait BT, Rout MP. Nat Methods, 2014 Dec;11(12):1253-60 PMID: 25362362; DOI: 10.1038/nmeth.3170
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Revision as of 10:10, 6 October 2018


anti-GFP (LaG16)

Anti-GFP(LaG16) is a readily expressible recombinant nanobody which has a high affinity and high specificity against GFP. It can be used as the extracellular domain of the SynNotch, thus accomplishing the contact-dependent signal input against GFP. When fused with LaG2(Click here to see!) using a flexible glycine-rich peptide linker to form a dimerization, ultra-high affinity can be conducted[1].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 243
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

[1]A robust pipeline for rapid production of versatile nanobody repertoires. Fridy PC, Li Y, Keegan S, ..., Chait BT, Rout MP. Nat Methods, 2014 Dec;11(12):1253-60 PMID: 25362362; DOI: 10.1038/nmeth.3170