Difference between revisions of "Part:BBa K2788002:Design"
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/mol_type="mRNA" | /mol_type="mRNA" | ||
/strain="ARSEF 23" | /strain="ARSEF 23" | ||
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===References=== | ===References=== | ||
Hu X, Xiao G, Zheng P, Shang Y, Su Y, Zhang X, Liu X, Zhan S, St Leger RJ, Wang C.Trajectory and genomic determinants of fungal-pathogen speciation and host adaptation.Proc Natl Acad Sci U S A. 2014 Nov 25; 111(47):16796-801. Epub 2014 Nov 3. | Hu X, Xiao G, Zheng P, Shang Y, Su Y, Zhang X, Liu X, Zhan S, St Leger RJ, Wang C.Trajectory and genomic determinants of fungal-pathogen speciation and host adaptation.Proc Natl Acad Sci U S A. 2014 Nov 25; 111(47):16796-801. Epub 2014 Nov 3. | ||
Wang C, St Leger RJ. A collagenous protective coat enables Metarhizium anisopliae to evade insect immune responses. Proc Natl Acad Sci USA. 2006;103(17):6647–6652. | Wang C, St Leger RJ. A collagenous protective coat enables Metarhizium anisopliae to evade insect immune responses. Proc Natl Acad Sci USA. 2006;103(17):6647–6652. | ||
Revision as of 10:03, 6 October 2018
Encoded by the gene MCL1 from Metarhizium robertsii ARSEF 23
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 157
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1688
Illegal NgoMIV site found at 1739
Illegal NgoMIV site found at 1864
Illegal AgeI site found at 1670 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1106
Illegal SapI site found at 1882
Illegal SapI site found at 1901
Design Notes
The studies on this protein are slim,there is no prepared antibody on sale.So if you want to study on its expression,for example,you want to do Western Blot and other itechnique based on antigen-antibody reaction,you'd better customize antibody in advance.Besides,preparation of histidine label fusion protein is an alternative proposal.
Source
/organism="Metarhizium robertsii" /mol_type="mRNA" /strain="ARSEF 23"
References
Hu X, Xiao G, Zheng P, Shang Y, Su Y, Zhang X, Liu X, Zhan S, St Leger RJ, Wang C.Trajectory and genomic determinants of fungal-pathogen speciation and host adaptation.Proc Natl Acad Sci U S A. 2014 Nov 25; 111(47):16796-801. Epub 2014 Nov 3.
Wang C, St Leger RJ. A collagenous protective coat enables Metarhizium anisopliae to evade insect immune responses. Proc Natl Acad Sci USA. 2006;103(17):6647–6652.