Difference between revisions of "Part:BBa K2788020:Design"
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===References=== | ===References=== | ||
| − | This part created by 2018 SZU-China iGEM team basing on the original part:BBa_K2232003 | + | This part created by 2018 SZU-China iGEM team basing on the original part:<partinfo>BBa_K2232003</partinfo> |
Aono R, Ito M, Machida T. Contribution of the Cell Wall Component Teichuronopeptide, to pH Homeostasis and Alkaliphily in the Alkaliphile, Bacillus lentus C-125[J]. Journal of Bacteriology, 1999, 181(21):6600-6. | Aono R, Ito M, Machida T. Contribution of the Cell Wall Component Teichuronopeptide, to pH Homeostasis and Alkaliphily in the Alkaliphile, Bacillus lentus C-125[J]. Journal of Bacteriology, 1999, 181(21):6600-6. | ||
Revision as of 06:24, 6 October 2018
C125-TupA-6His
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 969
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 789
Illegal BsaI.rc site found at 1181
Design Notes
In order to verify that TupA was indeed expressed at the protein level, 2018SZU-China added a 6-His structure to the original sequence and verified it by western blot.
Source
BBa_K2232003
References
This part created by 2018 SZU-China iGEM team basing on the original part:BBa_K2232003
Aono R, Ito M, Machida T. Contribution of the Cell Wall Component Teichuronopeptide, to pH Homeostasis and Alkaliphily in the Alkaliphile, Bacillus lentus C-125[J]. Journal of Bacteriology, 1999, 181(21):6600-6.