Difference between revisions of "Part:BBa K2547004"

 
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<partinfo>BBa_K2547004 short</partinfo>
 
<partinfo>BBa_K2547004 short</partinfo>
  
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The protein encoded by this gene is one of several isozymes of carbonic anhydrase, which catalyzes reversible hydration of carbon dioxide.We carried out site mutation on its DNA molecule and found that the mutant carbonic anhydrase had higher activity when the amino acid codon 203 encoded changed from leucine to lysine.
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This part is the coding sequence (CDS) of human carbonic anhydrase (CA-II), because the carbonic anhydrase (CA-II) extracted in human body has the fastest reaction rate at 37 ° C, and loses at 50 ° C. Live, so the DNA molecule was mutated and found that when the amino acid encoded by the 203th codon was mutated from leucine to lysine, the carbonic anhydrase produced had higher thermal stability and was encoded. The addition of a histidine tag (His-Tag) following the sequence (CDS) facilitates the purification of the carbonic anhydrase protein.
  
 
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Revision as of 03:34, 6 October 2018


Carbonic anhydrase 2 (L203K)

This part is the coding sequence (CDS) of human carbonic anhydrase (CA-II), because the carbonic anhydrase (CA-II) extracted in human body has the fastest reaction rate at 37 ° C, and loses at 50 ° C. Live, so the DNA molecule was mutated and found that when the amino acid encoded by the 203th codon was mutated from leucine to lysine, the carbonic anhydrase produced had higher thermal stability and was encoded. The addition of a histidine tag (His-Tag) following the sequence (CDS) facilitates the purification of the carbonic anhydrase protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]