Difference between revisions of "Part:BBa K2609026:Design"

(Design Notes)
(Source)
Line 12: Line 12:
 
===Source===
 
===Source===
  
-
+
Assembled using part <a href="https://parts.igem.org/Part:BBa_K1433005"> BBa_K14433005 </a> and a T7 adapter.
  
 
===References===
 
===References===

Revision as of 19:18, 5 October 2018


Lambda Red Recombinases under T7 expression system


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1943
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1460
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is under a T7 adapter having T7 promoter upstream and a T7 terminator downstream. It has HindIII and NheI sites on either sides which was used for easy cloning into the adapter.

Source

Assembled using part <a href="https://parts.igem.org/Part:BBa_K1433005"> BBa_K14433005 </a> and a T7 adapter.

References