Difference between revisions of "Part:BBa K2609013"
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Revision as of 18:48, 5 October 2018
gp37 under T7 expression system - Mod II
Coding sequence of the long tail fiber protein from T4 bacteriophage under the T7 expression system. The protein is fused to a 6xHis tag at the N-term through a enterokinase tag for easy purification. The sequence has been modified in-silico for better binding to phosphoethanolamine near the receptor binding end of the protein. This is the first such modification in our series of four modifications.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1239
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 507
- 1000COMPATIBLE WITH RFC[1000]
References
[1] Leiman, Petr G., et al. "Three-dimensional rearrangement of proteins in the tail of bacteriophage T4 on infection of its host." Cell 118.4 (2004): 419-429.
[2] Yoichi, Masatoshi, et al. "Alteration of tail fiber protein gp38 enables T2 phage to infect Escherichia coli O157: H7." Journal of biotechnology 115.1 (2005): 101-107.
[3] Rakhuba, D. V., et al. "Bacteriophage receptors, mechanisms of phage adsorption and penetration into host cell." Pol. J. Microbiol 59.3 (2010): 145-155.
[4] Bartual, Sergio G., et al. "Structure of the bacteriophage T4 long tail fiber receptor-binding tip." Proceedings of the National Academy of Sciences 107.47 (2010): 20287-20292.