Difference between revisions of "Part:BBa K2570021"

Revision as of 16:25, 5 October 2018

dsrA+B0034+GFP+B0032+mazF

Temperature is one environmental variable that all organisms must deal with. Besides the direct effect of temperature on enzymatic reactions, temperature response involves remodeling of the expression pattern of genes, affecting transcription, RNA stability, translation efficiency, and/or proteolysis (7, 8, 13, 20, 27). RpoS is a stationary-phase and stress response sigma factor in E. coli and many other bacteria. One environmental cue that increases RpoS synthesis is low temperature (below 37°C). This increase is completely dependent upon DsrA, a small noncoding RNA[2]. DsrA was shown to stimulate RpoS translation by pairing with a portion of the mRNA upstream of the RpoS translation start that can pair with and occlude the ribosome-binding site of the transcript [3]. DsrA is more abundant in E. coli at low growth temperatures than at higher temperatures, resulting in the increased RpoS expression at low temperatures [4]. Temperature affects both the synthesis and the stability of DsrA, leading to thermocontrol of RpoS translation.

Then,we use the GFP(B0040) to characterize the intensity of the temperature-controlled promoter.

Sequence and Features