Difference between revisions of "User:KCliff/Evaluating Cross Contamination Using The Pin Tool"

Line 5: Line 5:
 
Materials:  96-deep well plate           
 
Materials:  96-deep well plate           
 
             25% Cresol Red
 
             25% Cresol Red
             Pin Tool
+
             96 Multi-Blot Replicator (Pin Tool)
             Thesis Paper
+
             Thesis Paper with iGEM 96-block grid

Revision as of 17:59, 15 February 2008

In preparation for the 2008 iGEM competition, selected DNA 'bioparts' found in the Registry are transferred onto sheets of thesis paper using the pin tool in order to be mailed to teams at participating colleges and universities. Since this is the first year that iGEM will be using the thesis paper in place of actual plates to distribute the 'bioparts', the pin tool method of transfer was evaluated for possible DNA and culture cross contamination.

Cross Contamination Among DNA Samples from the Same Source Plate

Materials: 96-deep well plate

           25% Cresol Red
           96 Multi-Blot Replicator (Pin Tool)
           Thesis Paper with iGEM 96-block grid