Difference between revisions of "User:Msicchio"

Revision as of 17:49, 15 February 2008

Punch tool cleaning protocol

Pull the punch tool apart.

Dip both the rod and the column briefly into solutions of 10% bleach, distilled water, followed by second bath of distilled water, and a fourth bath of 95% ethanol. Ethanol is preferred as it dries much faster. Strong detergents should also be avoided.

Blot with a Kim wipe and allow to dry for 10 min. Note that the ethanol can go up inside the column of the punch tool, so it is best to blot until all liquid is gone. It is also important that the punch tool is completely dry before punching out another DNA spot, as the alcohol will effect the transformation efficiency.

TE Buffer for thesis paper punches

100 mmol Tris

5.1 mmol EDTA

can store paper either open or folded

@@ Line 3: / Line 3: @@
 Pull the punch tool apart.
-Dip three times into solutions of 10% bleach, distilled water, followed by second bath of distilled water, and 95% ethanol.  Ethanol dries much faster and Strong detergents should be avoided.
+Dip both the rod and the column briefly into solutions of 10% bleach, distilled water, followed by second bath of distilled water, and a fourth bath of 95% ethanol.  Ethanol is preferred as it dries much faster. Strong detergents should also be avoided.
-Blot with a Kim wipe and allow to dry for 10 min. Note that the ethanol can go up into the punch tool, so it is best to blot  It is also important that the punch tool is completely dry before punching out another DNA spot, as the alcohol will effect the transformation efficiency.
+Blot with a Kim wipe and allow to dry for 10 min. Note that the ethanol can go up inside the column of the punch tool, so it is best to blot until all liquid is gone.  It is also important that the punch tool is completely dry before punching out another DNA spot, as the alcohol will effect the transformation efficiency.
 == TE Buffer for thesis paper punches==