Difference between revisions of "Part:BBa I744121:Experience"

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===User Reviews===
 
===User Reviews===
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Part is designated as having issues because it appears to be lethal in cells containing the Tn10 transposon.  The hypothesis is that the ptet promoter located on the high copy number plasmid "pulls" TetR created by the chromosomally encoded Tn10 transposon, causing massive derepression of TetA efflux pump, which is lethal in high levels.   
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Part is designated as having issues because it appears to be lethal in cells containing the Tn10 transposon. The hypothesis is that the ptet promoter located on the high copy number plasmid "pulls" TetR created by the chromosomally encoded Tn10 transposon, causing massive derepression of TetA efflux pump, which is lethal in high levels.   
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Enter the review information here.
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Latest revision as of 21:21, 8 February 2008


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_I744121

User Reviews

Part is designated as having issues because it appears to be lethal in cells containing the Tn10 transposon. The hypothesis is that the ptet promoter located on the high copy number plasmid "pulls" TetR created by the chromosomally encoded Tn10 transposon, causing massive derepression of TetA efflux pump, which is lethal in high levels.

UNIQ93643ca5ee19e1d0-partinfo-00000000-QINU UNIQ93643ca5ee19e1d0-partinfo-00000001-QINU