Difference between revisions of "Part:BBa K2623015:Experience"
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===Applications of BBa_K2623015=== | ===Applications of BBa_K2623015=== | ||
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In our circuit of the build process, we have been doing nucleic acid gel electrophoresis to verify. After the loop is complete, sequencing verification.<br> | In our circuit of the build process, we have been doing nucleic acid gel electrophoresis to verify. After the loop is complete, sequencing verification.<br> | ||
https://static.igem.org/mediawiki/parts/1/1c/SAHS_1_Fig1.png<br> | https://static.igem.org/mediawiki/parts/1/1c/SAHS_1_Fig1.png<br> | ||
Revision as of 10:42, 5 October 2018
Applications of BBa_K2623015
Identification
In our circuit of the build process, we have been doing nucleic acid gel electrophoresis to verify. After the loop is complete, sequencing verification.
After the fluorescence identification, we performed a small amount of protein expression by SDS-PAGE.
SAHS is an exogenous protein with a signal peptide in front of it that allows the protein to be secreted outside the membrane. But our site organism is E. coli, because there is a cell wall, so the protein can not be secreted out of the membrane. Therefore, we can hardly see our target protein in the supernatant. Therefore, we can only obtain the protein we need from the cell pellet. After that, we connected our SAHS protein to the pET-28a plasmid, induced expression, and obtained a large amount of our target protein by ultrasonic disruption of the bacterial cell pellet.
More information about our project can be found on our results page.
User Reviews
UNIQ6855279d7d4bc524-partinfo-00000000-QINU UNIQ6855279d7d4bc524-partinfo-00000001-QINU