Difference between revisions of "Part:BBa R0040"
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Sequence for pTet inverting regulator driven by the TetR protein. | Sequence for pTet inverting regulator driven by the TetR protein. | ||
| − | + | Promoter is constitutively ON and repressed by TetR. TetR repression is inhibited by the addition of tetracycline or its analog, [http://openwetware.org/wiki/ATc aTc]. | |
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 19:53, 8 February 2008
TetR repressible promoter
Sequence for pTet inverting regulator driven by the TetR protein. Promoter is constitutively ON and repressed by TetR. TetR repression is inhibited by the addition of tetracycline or its analog, [http://openwetware.org/wiki/ATc aTc].
Usage and Biology
Medium strength promoter. [jb, 5/24/04]
From the reference article:"In contrast to tetracycline, anhydrotetracycline is a particularly useful inducer. It binds Tet R with an ~35-fold higher binding constant and thus allows to operate at very low concentrations. At the same time, its antibiotic activity is ~100-fold lower and concentrations of <50 ng/ml as required for the full induction of P LtetO-1 have no effect on the growth of E.coli."
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]