Difference between revisions of "Part:BBa K2570002:Design"

(Design Notes)
(Design Notes)
 
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===Design Notes===
 
===Design Notes===
Site-directed mutation site
+
<span style="font-weight:bold;">Site-directed mutation site </span>
  
 
TyrB(Pst1:CTGCAG—CTGCAA)
 
TyrB(Pst1:CTGCAG—CTGCAA)

Latest revision as of 09:34, 5 October 2018


TyrB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1054
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Site-directed mutation site

TyrB(Pst1:CTGCAG—CTGCAA)

sense primer: GGTGGCTGCAATGCTGAATGACG

Anti-sense primer: TCAGCATTGCAGCCACCACCTG

Source

Phenylalanine aminotransferase Tyrb from Escherichia coli 21B.

https://www.ncbi.nlm.nih.gov/protein/WP_032305522.1?report=genbank&log$=protalign&blast_rank=3&RID=V13P17MW014

References