Difference between revisions of "Part:BBa P10101:Experience"
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===Applications of BBa_P10101=== | ===Applications of BBa_P10101=== | ||
− | The Cardiff iGEM team of 2018 characterised this promoter using the GUS and mCherry reporter genes. We compared the expression of this promoter to that of the [https://parts.igem.org/Part:BBa_K2810002 RTBV promoter] using the same reporter genes. These can be seen below. We found that using mCherry as a reporter gene, we could quantify the expression of the reporter, and found that it increased fluorescence to 1000x negative control levels, and between 10-100x that of RTBV levels. | + | The Cardiff iGEM team of 2018 characterised this promoter using the [https://parts.igem.org/Part:BBa_K2810001 GUS] and [https://parts.igem.org/Part:BBa_K2810009 mCherry] reporter genes. We compared the expression of this promoter to that of the [https://parts.igem.org/Part:BBa_K2810002 RTBV promoter] using the same reporter genes. These can be seen below. We found that using mCherry as a reporter gene, we could quantify the expression of the reporter, and found that it increased fluorescence to 1000x negative control levels, and between 10-100x that of RTBV levels. |
Revision as of 09:07, 5 October 2018
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Applications of BBa_P10101
The Cardiff iGEM team of 2018 characterised this promoter using the GUS and mCherry reporter genes. We compared the expression of this promoter to that of the RTBV promoter using the same reporter genes. These can be seen below. We found that using mCherry as a reporter gene, we could quantify the expression of the reporter, and found that it increased fluorescence to 1000x negative control levels, and between 10-100x that of RTBV levels.
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UNIQ3b724991b3bb84b6-partinfo-00000000-QINU UNIQ3b724991b3bb84b6-partinfo-00000001-QINU