Difference between revisions of "Part:BBa P10101"
 
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PhytoBricks part from plasmid pUAP41373
 
PhytoBricks part from plasmid pUAP41373
  
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The Cardiff iGEM team of 2018 characterised this promoter using the GUS and mCherry reporter genes. We compared the expression of this promoter to that of the [https://parts.igem.org/Part:BBa_K2810002 RTBV promoter] using the same reporter genes. These can be seen below. We found that using mCherry as a reporter gene, we could quantify the expression of the reporter, and found that it increased fluorescence to 1000x negative control levels, and between 10-100x that of RTBV levels.
 
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[[File:https://static.igem.org/mediawiki/2018/6/6f/T--Cardiff_Wales--PromoterResults.png|700px|thumb|left|Figure 1) See only the GUS and mCherry constructs labelled 35S-OTMV-GUS-NosT, or 35S-OTMV-mCherry-NosT]]
 
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[[File:https://static.igem.org/mediawiki/2018/1/19/T--Cardiff_Wales--mCherryPromoterAssayQuantified.png|700px|thumb|left|Figure 2)
 
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The quantification report of the 35S promoter when linked to mCherry. This shows the raw photon output of leaf tissue.]]
 
  
  

Latest revision as of 08:31, 5 October 2018


35s cauliflower mosaic virus

PhytoBricks part from plasmid pUAP41373




Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 12
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 12
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 12
    Illegal BglII site found at 399
    Illegal BglII site found at 1145
    Illegal XhoI site found at 1352
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 12
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 12
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 1355