Difference between revisions of "Part:BBa J45993:Design"

(Design Notes)
(Source)
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===Source===
 
===Source===
  
This part was PCRed out of E. coli's genome with the primers listed in the Design Notes section.
+
This part was constructed from the annealing of the primers listed in the Design Notes section.
  
 
===References===
 
===References===

Revision as of 19:57, 4 February 2008


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Design Notes

Forward Primer

5'-CTA GAG GCT TAT GTT TTC GCT GAT ATC CCG AGC GGT TTC AAA ATT GTG ATC TAT ATT TAA CAA ATA CTA GTA GCG GCC GCT GCA-3'

Reverse Primer

5'-GCG GCC GCT ACT AGT ATT TGT TAA ATA TAG ATC ACA ATT TTG AAA CCG CTC GGG ATA TCA GCG AAA ACA TAA GCC T-3'

Source

This part was constructed from the annealing of the primers listed in the Design Notes section.

References

<biblio>

  1. Yim pmid=8282684
  2. Schellhorn pmid=9829938
  3. Wise pmid=8631665
  4. Funabashi pmid=11879714
  5. Makinoshima pmid=11985708

</biblio>

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=453110 S67278]

Neidhart, Frederick C. Escherichia coli and Salmonella. Washington: ASM Press, 1996.