Difference between revisions of "Part:BBa K2833013"

 
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<partinfo>BBa_K2833013 short</partinfo>
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Ice nucleation protein(INP)is a secretory outer membrane protein from Pseudomomas syringae，P． flurorescens and several other Gram—negative bacteria．Using its characteristic of forming ice nucleation at relatively warm temperatures(一2～一4‘C)，INP is originally applied in biological refrigeration field．In the bacterial surface display technology，INP is widely used as a functional carrier protein．Compared with other carrier proteins，INP has advantages such as stably expressing foreign proteins and showing the larger molecular weight proteins．INP cell—sur— face display technology has been developed tO be a promising approach in many biotechn0109ical fields，such as whole cell bioeatalysts，whole cell bioadsorbence and biodegradation of environmental pollutants．
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Vitreoscilla haemoglobin (VHb) is a type of haemoglobin found in the Gram-negative aerobic bacterium, Vitreoscilla. It is the first haemoglobin discovered from bacteria, but unlike classic hemoglobin it is composed only of a single globin molecule.
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2833013 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K2833013 parameters</partinfo>
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Revision as of 07:28, 5 October 2018


J23104+B0032+INP+VHb+Ter

Ice nucleation protein(INP)is a secretory outer membrane protein from Pseudomomas syringae，P． flurorescens and several other Gram—negative bacteria．Using its characteristic of forming ice nucleation at relatively warm temperatures(一2～一4‘C)，INP is originally applied in biological refrigeration field．In the bacterial surface display technology，INP is widely used as a functional carrier protein．Compared with other carrier proteins，INP has advantages such as stably expressing foreign proteins and showing the larger molecular weight proteins．INP cell—sur— face display technology has been developed tO be a promising approach in many biotechn0109ical fields，such as whole cell bioeatalysts，whole cell bioadsorbence and biodegradation of environmental pollutants．

Vitreoscilla haemoglobin (VHb) is a type of haemoglobin found in the Gram-negative aerobic bacterium, Vitreoscilla. It is the first haemoglobin discovered from bacteria, but unlike classic hemoglobin it is composed only of a single globin molecule. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1451
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 467
  • 1000
    COMPATIBLE WITH RFC[1000]