Difference between revisions of "Part:BBa P10101"
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| − | [[ | + | [[File:https://static.igem.org/mediawiki/2018/6/6f/T--Cardiff_Wales--PromoterResults.png|700px|thumb|left|Figure 1) See only the GUS and mCherry constructs labelled 35S-OTMV-GUS-NosT, or 35S-OTMV-mCherry-NosT]] |
Revision as of 22:25, 4 October 2018
35s cauliflower mosaic virus
PhytoBricks part from plasmid pUAP41373
The Cardiff iGEM team of 2018 characterised this promoter using the GUS and mCherry reporter genes. We compared the expression of this promoter to that of the RTBV promoter using the same reporter genes. These can be seen below. We found that using mCherry as a reporter gene, we could quantify the expression of the reporter, and found that it increased fluorescence to 1000x negative control levels, and between 10-100x that of RTBV levels.
File:Https://static.igem.org/mediawiki/2018/6/6f/T--Cardiff Wales--PromoterResults.png
Figure 1) See only the GUS and mCherry constructs labelled 35S-OTMV-GUS-NosT, or 35S-OTMV-mCherry-NosT
File:Https://static.igem.org/mediawiki/2018/1/19/T--Cardiff Wales--mCherryPromoterAssayQuantified.png
Figure 2) The quantification report of the 35S promoter when linked to mCherry. This shows the raw photon output of leaf tissue.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 12
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 12
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 12
Illegal BglII site found at 399
Illegal BglII site found at 1145
Illegal XhoI site found at 1352 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 12
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 12
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1
Illegal BsaI.rc site found at 1355