Difference between revisions of "Part:BBa K2555003"
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  BBa_K2555003 contains PBAD promoter, RBS, and superfolder GFP. The construct is used to indirectly reflect the expression of cueR of BBa_K2555002 under different concentration of L-arabinose by measuring green fluorescent intensity with plate reader. BBa_K2555002 contains pBAD (L-arabinose inducing promoter) with RBS and CueR ( a transcription factor that can bind on pcopA of BBa_K2555000). E. coli cells use CueR to regulate the cytoplasmic copper concentration. Arabinose concentration controls the expression of CueR in the construct. CueR behaves as a net activator or a net repressor under different copper concentrations(1). BBa_K2555002 and  BBa_K2555000 are used for construction of BBa_K2555004.
 
  BBa_K2555003 contains PBAD promoter, RBS, and superfolder GFP. The construct is used to indirectly reflect the expression of cueR of BBa_K2555002 under different concentration of L-arabinose by measuring green fluorescent intensity with plate reader. BBa_K2555002 contains pBAD (L-arabinose inducing promoter) with RBS and CueR ( a transcription factor that can bind on pcopA of BBa_K2555000). E. coli cells use CueR to regulate the cytoplasmic copper concentration. Arabinose concentration controls the expression of CueR in the construct. CueR behaves as a net activator or a net repressor under different copper concentrations(1). BBa_K2555002 and  BBa_K2555000 are used for construction of BBa_K2555004.
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===Usage and Biology===
 
===Usage and Biology===

Revision as of 14:35, 4 October 2018


pBAD-RBS-sfGFP 

BBa_K2555003 contains PBAD promoter, RBS, and superfolder GFP. The construct is used to indirectly reflect the expression of cueR of BBa_K2555002 under different concentration of L-arabinose by measuring green fluorescent intensity with plate reader. BBa_K2555002 contains pBAD (L-arabinose inducing promoter) with RBS and CueR ( a transcription factor that can bind on pcopA of BBa_K2555000). E. coli cells use CueR to regulate the cytoplasmic copper concentration. Arabinose concentration controls the expression of CueR in the construct. CueR behaves as a net activator or a net repressor under different copper concentrations(1). BBa_K2555002 and  BBa_K2555000 are used for construction of BBa_K2555004.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1311
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1250
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1085
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1067
    Illegal SapI.rc site found at 1355


References

(1)Danya J. Martell, Chandra P. Joshi, Ahmed Gaballa, Ace George Santiago, Tai-Yen Chen, Won Jung, John D. Helmann, and Peng Chen (2015) “Metalloregulator CueR biases RNA polymerase’s kinetic sampling of dead-end or open complex to repress or activate transcription” Proc Natl Acad Sci U S A. 2015 Nov 3; 112(44): 13467–13472.