Difference between revisions of "Part:BBa K2555003"
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<partinfo>BBa_K2555003 short</partinfo> | <partinfo>BBa_K2555003 short</partinfo> | ||
| − | BBa_K2555002 contains pBAD (L-arabinose inducing promoter) with RBS and CueR ( a transcription factor that can bind on pcopA of BBa_K2555000). E. coli cells use CueR to regulate the cytoplasmic copper concentration. Arabinose concentration controls the expression of CueR in the construct. CueR behaves as a net activator or a net repressor under different copper concentrations(1). BBa_K2555002 and BBa_K2555000 are used for construction of BBa_K2555004. | + | BBa_K2555003 contains PBAD promoter, RBS, and superfolder GFP. The construct is used to indirectly reflect the expression of cueR of BBa_K2555002 under different concentration of L-arabinose by measuring green fluorescent intensity with plate reader. BBa_K2555002 contains pBAD (L-arabinose inducing promoter) with RBS and CueR ( a transcription factor that can bind on pcopA of BBa_K2555000). E. coli cells use CueR to regulate the cytoplasmic copper concentration. Arabinose concentration controls the expression of CueR in the construct. CueR behaves as a net activator or a net repressor under different copper concentrations(1). BBa_K2555002 and BBa_K2555000 are used for construction of BBa_K2555004. |
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===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 14:35, 4 October 2018
pBAD-RBS-sfGFP
BBa_K2555003 contains PBAD promoter, RBS, and superfolder GFP. The construct is used to indirectly reflect the expression of cueR of BBa_K2555002 under different concentration of L-arabinose by measuring green fluorescent intensity with plate reader. BBa_K2555002 contains pBAD (L-arabinose inducing promoter) with RBS and CueR ( a transcription factor that can bind on pcopA of BBa_K2555000). E. coli cells use CueR to regulate the cytoplasmic copper concentration. Arabinose concentration controls the expression of CueR in the construct. CueR behaves as a net activator or a net repressor under different copper concentrations(1). BBa_K2555002 and BBa_K2555000 are used for construction of BBa_K2555004.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1311
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1250
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1085
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1067
Illegal SapI.rc site found at 1355
References
(1)Danya J. Martell, Chandra P. Joshi, Ahmed Gaballa, Ace George Santiago, Tai-Yen Chen, Won Jung, John D. Helmann, and Peng Chen (2015) “Metalloregulator CueR biases RNA polymerase’s kinetic sampling of dead-end or open complex to repress or activate transcription” Proc Natl Acad Sci U S A. 2015 Nov 3; 112(44): 13467–13472.