Difference between revisions of "Part:BBa K2555002"

 
Line 2: Line 2:
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K2555002 short</partinfo>
 
<partinfo>BBa_K2555002 short</partinfo>
−
 
+
This construct contains pBAD (L-arabinose inducing promoter) with RBS and CueR (a transcription factor that binds onto pCopA). CueR under the control of the promoter it regulates would produce a positive feedback loop, making the construct more sensitive to different copper concentrations.
−
BBa_K2555002 including araC, the promoter of pBAD, ribosome binding site, and CueR. In E.coli. arabinose combined with araC which can active pBAD promoter. And the pBAD promoter will generate CueR.  
+
CueR can act as both a positive and negative regulator and our results seem to indicate that negative regulation may be occurring here.
−
 
+
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 10:04, 4 October 2018


pBAD-RBS-CueR This construct contains pBAD (L-arabinose inducing promoter) with RBS and CueR (a transcription factor that binds onto pCopA). CueR under the control of the promoter it regulates would produce a positive feedback loop, making the construct more sensitive to different copper concentrations. CueR can act as both a positive and negative regulator and our results seem to indicate that negative regulation may be occurring here. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1311
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1250
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1085
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1067