Difference between revisions of "Part:BBa K2598025"
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<partinfo>BBa_K2598025 short</partinfo> | <partinfo>BBa_K2598025 short</partinfo> | ||
| − | This part contains lacZ under a CGG-regulated promoter pCGG, T7 RNAP sigma fragment CGG(BBa_K2598011) can direct T7 core fragment to pCGG, | + | This part contains lacZ under a CGG-regulated promoter pCGG, T7 RNAP sigma fragment CGG(BBa_K2598011) can direct T7 core fragment to pCGG, inducing the expression of downstream genes. A strong terminator is added to avoid recombination. |
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Revision as of 04:42, 4 October 2018
lacZ under CGG-regulated promoter pCGG
This part contains lacZ under a CGG-regulated promoter pCGG, T7 RNAP sigma fragment CGG(BBa_K2598011) can direct T7 core fragment to pCGG, inducing the expression of downstream genes. A strong terminator is added to avoid recombination.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 3112
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 5
- 1000COMPATIBLE WITH RFC[1000]