Difference between revisions of "Part:BBa K2549010"

 
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<partinfo>BBa_K2549010 short</partinfo>
  
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This part is the C-terminal fragment of Cfa. Cfa is a consensus sequence from an alignment of 73 naturally occurring DnaE inteins that are predicted to have fast splicing rates. Cfa demonstrates both rapid protein splicing and unprecedented thermal and chaotropic durability. The 122-124 residues of Cfa is mutated from EKD to GEP, which has been proved to imbue ultrafast DnaE split inteins with minimal extein dependence, thus improving split Intein-mediated protein cyclization. Cfa’C is used in our amplifier to accomplish some complex logic functions. It can also be used by other iGEM teams to assembly their intein-based protein libraries.
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2549010 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K2549010 parameters</partinfo>
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Revision as of 12:57, 3 October 2018

split intein Cfa C

This part is the C-terminal fragment of Cfa. Cfa is a consensus sequence from an alignment of 73 naturally occurring DnaE inteins that are predicted to have fast splicing rates. Cfa demonstrates both rapid protein splicing and unprecedented thermal and chaotropic durability. The 122-124 residues of Cfa is mutated from EKD to GEP, which has been proved to imbue ultrafast DnaE split inteins with minimal extein dependence, thus improving split Intein-mediated protein cyclization. Cfa’C is used in our amplifier to accomplish some complex logic functions. It can also be used by other iGEM teams to assembly their intein-based protein libraries.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 91
    Illegal SapI.rc site found at 21