Difference between revisions of "Part:BBa K2810000:Experience"

 
 
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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how you used this part and how it worked out.
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We assembled this part into a level 1 composite part designed to express a transgene designed for an RNAi experiment ([https://parts.igem.org/Part:BBa_P10101 35S]-[https://parts.igem.org/Part:BBa_K2810005 BCR3]-[https://parts.igem.org/Part:BBa_K2810000 WRKYintron]-[https://parts.igem.org/Part:BBa_K2810006 BCR*]-[https://parts.igem.org/Part:BBa_P10401 NosT]). This was transiently expressed in tobacco leaves. We aimed to identify expression of this clone using RT-PCR. However we obtained inconclusive results when we attempted to assess levels of expression.
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https://static.igem.org/mediawiki/2018/thumb/c/ce/T--Cardiff_Wales--BCR3_RT-PCR.png/800px-T--Cardiff_Wales--BCR3_RT-PCR.png
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These experiments are outlined on the [http://2018.igem.org/Team:Cardiff_Wales/Results Cardiff_Wales 2018 Results page].
  
 
===Applications of BBa_K2810000===
 
===Applications of BBa_K2810000===

Latest revision as of 12:46, 3 October 2018



We assembled this part into a level 1 composite part designed to express a transgene designed for an RNAi experiment (35S-BCR3-WRKYintron-BCR*-NosT). This was transiently expressed in tobacco leaves. We aimed to identify expression of this clone using RT-PCR. However we obtained inconclusive results when we attempted to assess levels of expression.
800px-T--Cardiff_Wales--BCR3_RT-PCR.png
These experiments are outlined on the [http://2018.igem.org/Team:Cardiff_Wales/Results Cardiff_Wales 2018 Results page].

Applications of BBa_K2810000

User Reviews

UNIQ3c491b6c30f1ebe1-partinfo-00000000-QINU UNIQ3c491b6c30f1ebe1-partinfo-00000001-QINU