Difference between revisions of "Part:BBa K2810006:Experience"

 
 
Line 1: Line 1:
  
 
__NOTOC__
 
__NOTOC__
−
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
+
<br>
−
how you used this part and how it worked out.
+
We assembled this part into a level 1 composite part designed to express a transgene designed for an RNAi experiment ([https://parts.igem.org/Part:BBa_P10101 35S]-[https://parts.igem.org/Part:BBa_K2810005 BCR3]-[https://parts.igem.org/Part:BBa_K2810000 WRKYintron]-[https://parts.igem.org/Part:BBa_K2810006 BCR*]-[https://parts.igem.org/Part:BBa_P10401 NosT]). This was transiently expressed in tobacco leaves. We aimed to identify expression of this clone using RT-PCR. However we obtained inconclusive results when we attempted to assess levels of expression.
 +
<br>
 +
https://static.igem.org/mediawiki/2018/thumb/c/ce/T--Cardiff_Wales--BCR3_RT-PCR.png/800px-T--Cardiff_Wales--BCR3_RT-PCR.png
 +
<br>
 +
These experiments are outlined on the [http://2018.igem.org/Team:Cardiff_Wales/Results Cardiff_Wales 2018 Results page].
  
 
===Applications of BBa_K2810006===
 
===Applications of BBa_K2810006===

Latest revision as of 12:45, 3 October 2018



We assembled this part into a level 1 composite part designed to express a transgene designed for an RNAi experiment (35S-BCR3-WRKYintron-BCR*-NosT). This was transiently expressed in tobacco leaves. We aimed to identify expression of this clone using RT-PCR. However we obtained inconclusive results when we attempted to assess levels of expression.
800px-T--Cardiff_Wales--BCR3_RT-PCR.png
These experiments are outlined on the [http://2018.igem.org/Team:Cardiff_Wales/Results Cardiff_Wales 2018 Results page].

Applications of BBa_K2810006

User Reviews

UNIQ1d77ac65cf44965d-partinfo-00000000-QINU UNIQ1d77ac65cf44965d-partinfo-00000001-QINU