Difference between revisions of "Part:BBa K2549009"

 
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__NOTOC__
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<partinfo>BBa_K2549009 short</partinfo>
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This part is the N-terminal fragment of Cfa. Cfa is a consensus sequence from an alignment of 73 naturally occurring DnaE inteins that are predicted to have fast splicing rates. Cfa demonstrates both rapid protein splicing and unprecedented thermal and chaotropic durability. Besides, when the Cfa’N is fused to other proteins, it exhibits higher expression levels than other N-terminal fusions. Cfa’N is used in our amplifier to accomplish some complex logic functions. It can also be used by other iGEM teams to assembly their intein-based protein libraries.
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2549009 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K2549009 parameters</partinfo>
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Revision as of 10:26, 3 October 2018


split intein Cfa N

This part is the N-terminal fragment of Cfa. Cfa is a consensus sequence from an alignment of 73 naturally occurring DnaE inteins that are predicted to have fast splicing rates. Cfa demonstrates both rapid protein splicing and unprecedented thermal and chaotropic durability. Besides, when the Cfa’N is fused to other proteins, it exhibits higher expression levels than other N-terminal fusions. Cfa’N is used in our amplifier to accomplish some complex logic functions. It can also be used by other iGEM teams to assembly their intein-based protein libraries.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]