Difference between revisions of "Part:BBa K2708003:Design"
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When the circuit is activated by the promoter, the terminating substructure of the SAM ribose switch cannot be formed, making the circuit express GFP fluorescent protein downward. When the small molecule SAM binds to the SAM ribose switch, the terminating substructure of the SAM ribose switch is formed, making the circuit terminating and unable to express fluorescent protein. | When the circuit is activated by the promoter, the terminating substructure of the SAM ribose switch cannot be formed, making the circuit express GFP fluorescent protein downward. When the small molecule SAM binds to the SAM ribose switch, the terminating substructure of the SAM ribose switch is formed, making the circuit terminating and unable to express fluorescent protein. | ||
Latest revision as of 07:59, 3 October 2018
Promoter and S-adenosyl methionine riboswitch, GFP fluorescent expression system.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 963
===Design Notes==='
When the circuit is activated by the promoter, the terminating substructure of the SAM ribose switch cannot be formed, making the circuit express GFP fluorescent protein downward. When the small molecule SAM binds to the SAM ribose switch, the terminating substructure of the SAM ribose switch is formed, making the circuit terminating and unable to express fluorescent protein.
Source
Synthesis Riboswitch is a kind of RNA sequence element with special spatial structure. It is mainly found in the non-coding region of bacterial mRNA. SAM riboswitch is widely distributed in various bacterial lineages.