Difference between revisions of "Part:BBa K2467002:Experience"
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Five different concentrations of NaCl were added to tryptone and yeast extract (same as LB) and E.coli containing the biosensor plasmid and were grown overnight in triplicate. After 18 hours the red fluorescence of 100ul of culture was measured in a TECAN m1000 plate reader set for excitation/emission wavelengths of 584/607nm at a gain of 100. | Five different concentrations of NaCl were added to tryptone and yeast extract (same as LB) and E.coli containing the biosensor plasmid and were grown overnight in triplicate. After 18 hours the red fluorescence of 100ul of culture was measured in a TECAN m1000 plate reader set for excitation/emission wavelengths of 584/607nm at a gain of 100. | ||
| − | <img src="T--Pittsburgh_CSL--ProU_promoter_RFP_fluorescence.jpg"> | + | <img src="https://static.igem.org/mediawiki/2018/f/f8/T--Pittsburgh_CSL--ProU_promoter_RFP_fluorescence.jpg"> |
Figure showing fluorescence readings of RFP reporter for ProU consensus promoter. Error bars show standard error of the mean. | Figure showing fluorescence readings of RFP reporter for ProU consensus promoter. Error bars show standard error of the mean. | ||
Revision as of 18:19, 2 October 2018
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Applications of BBa_K2467002
Five different concentrations of NaCl were added to tryptone and yeast extract (same as LB) and E.coli containing the biosensor plasmid and were grown overnight in triplicate. After 18 hours the red fluorescence of 100ul of culture was measured in a TECAN m1000 plate reader set for excitation/emission wavelengths of 584/607nm at a gain of 100.
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Figure showing fluorescence readings of RFP reporter for ProU consensus promoter. Error bars show standard error of the mean.
These results show that the biosensor is sensitive from at low osmolarity (0-100mM NaCl). At more normal osmolarity (100-400mM NaCl) there is no difference (normal LB is 170mM NaCl).
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