Difference between revisions of "Part:BBa K2797003"
Msburridge (Talk | contribs) (→Newcastle 2018 - Interlab Characterisation) |
Msburridge (Talk | contribs) (→Newcastle 2018 - Interlab Characterisation) |
||
| Line 11: | Line 11: | ||
===Newcastle 2018 - Interlab Characterisation=== | ===Newcastle 2018 - Interlab Characterisation=== | ||
| + | |||
| + | [[Image:BBa_K2797003.png|center]] | ||
<!-- --> | <!-- --> | ||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K2797003 SequenceAndFeatures</partinfo> | <partinfo>BBa_K2797003 SequenceAndFeatures</partinfo> | ||
| − | + | ||
| + | |||
| + | |||
| + | |||
Revision as of 15:55, 2 October 2018
mNeonGreen
BBa_K2797003 (mNeonGreen) codon optimised for expression in E. Coli to replace mut3GFP as an improved fluorescent reporter in the interlab study.
Usage and Biology
The mNeonGreen protein is widely used in the imaging of cellular components due to it having a fluorescence 3-5 times that of GFP. Importantly however, it is thought to be more photostable than the mut3GFP used in the InterLab study, although there is little indication in the literature that it has been used as a reporter for the characterisation of circuits. Replacing mut3GFP with mNeonGreen allowed the investigation of whether the fast folding capabilities coupled with its brightness and higher photostability could yield a lower spread of fluorescence values in regard to the original mut3GFP, making it a better tool for part characterisation.
Newcastle 2018 - Interlab Characterisation
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 39
- 1000COMPATIBLE WITH RFC[1000]