Difference between revisions of "Part:BBa K2797002"

Revision as of 13:54, 2 October 2018

Composite part for streptomycin resistance

This composite part confers resistance to streptomycin. The part contains the constitutive BBa_J23119 promoter, the strongest promoter of the J23100 through to J23119 promoter family

Part Characterisation

Figure 1 E. coli strain DH5α transformed with streptomycin resistance part BBa_K2797002 growing on LB agar containing streptomycin at a concentration of 50 μg/ml.

Figure 2 Untransformed E. coli strain DH5α unable to grow on LB agar containing streptomycin at a concentration of 50 μg/ml.

Figure 3 E. coli strain DH5α transformed with streptomycin resistance part BBa_K2797002 growing on LB agar containing chloramphenicol at a concentration of 25 μg/ml due to resistance gene in pSB1C3 vector.

Figure 4 Optical density at 600nm of three replicate wells containing LB and incrementally increasing streptomycin concentrations inoculated with E. coli strain DH5α untransformed and transformed with part BBa_K2797002 over incubation at 37 C over 24 hours. Error bars show standard error mean.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 861
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 709
1000
COMPATIBLE WITH RFC[1000]

@@ Line 8: / Line 8: @@
 [[File:T--NEWCASTLE--STREPTOMYCIN ECOLISMR.jpeg|500px|]]
-'''Figure 1''' <I> E. coli </I> strain DH5α transformed with streptomycin resistance part BBa_K2797002 growing on LB agar containing streptomycin at a concentration of 50 μg/ml
+'''Figure 1''' <I> E. coli </I> strain DH5α transformed with streptomycin resistance part BBa_K2797002 growing on LB agar containing streptomycin at a concentration of 50 μg/ml.
 [[File:T--NEWCASTLE--STREPTOMYCIN ECOLI.jpeg|500px|]]
-'''Figure 2''' Untransformed <I> E. coli </I> strain DH5α unable to grow on LB agar containing streptomycin at a concentration of 50 μg/ml
+'''Figure 2''' Untransformed <I> E. coli </I> strain DH5α unable to grow on LB agar containing streptomycin at a concentration of 50 μg/ml.
 [[File:T--NEWCASTLE--SMRCMA.jpeg|500px|]]
-'''Figure 3''' <I> E. coli </I> strain DH5α transformed with streptomycin resistance part BBa_K2797002 growing on LB agar containing chloramphenicol at a concentration of 25 μg/ml due to resistance gene in pSB1C3 vector
+'''Figure 3''' <I> E. coli </I> strain DH5α transformed with streptomycin resistance part BBa_K2797002 growing on LB agar containing chloramphenicol at a concentration of 25 μg/ml due to resistance gene in pSB1C3 vector.
 [[File:T--NEWCASTLE--Strep.jpeg|900px|]]
-'''Figure 4''' Optical density at 600nm of three replicate wells containing LB with incrementally increasing streptomycin concentrations inoculated with <I>E. coli</I> strain DH5α untransformed and transformed with part BBa_K2797002 . Error bars show standard error mean
+'''Figure 4''' Optical density at 600nm of three replicate wells containing LB and incrementally increasing streptomycin concentrations inoculated with <I>E. coli</I> strain DH5α untransformed and transformed with part BBa_K2797002 over incubation at 37 C over 24 hours. Error bars show standard error mean.
 <!-- Add more about the biology of this part here