Difference between revisions of "Part:BBa K2623023"

 
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<partinfo>BBa_K2623023 short</partinfo>
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It’s another form of the BBa_K2623001.This part was designed to target our archaeal ribosomal protein protein L7Ae and then siRNA into outer-membrane vesicles (OMVs). Porin outer-membrane protein A, OmpA, is a kind of highly expressed transmembrane porin protein of E. coli and is therefore abundant in OMVs. We inserted SpyTag to its N-termini and SpyCather to L7Ae (BBa_K2623026) in order to anchore L7Ae to outer membrane through the bioconjugation of the SpyTag/SpyCatcher system. Through the fluorescence of GFP, we can evaluate the efficiency of their transport to the outer membrane and OMVs.
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2623022 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K2623022 parameters</partinfo>
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Revision as of 13:32, 2 October 2018


Bacterial outer membrane protein A (OmpA) fused with SpyTag and GFP at its N-termini

It’s another form of the BBa_K2623001.This part was designed to target our archaeal ribosomal protein protein L7Ae and then siRNA into outer-membrane vesicles (OMVs). Porin outer-membrane protein A, OmpA, is a kind of highly expressed transmembrane porin protein of E. coli and is therefore abundant in OMVs. We inserted SpyTag to its N-termini and SpyCather to L7Ae (BBa_K2623026) in order to anchore L7Ae to outer membrane through the bioconjugation of the SpyTag/SpyCatcher system. Through the fluorescence of GFP, we can evaluate the efficiency of their transport to the outer membrane and OMVs.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 65
  • 1000
    COMPATIBLE WITH RFC[1000]