Difference between revisions of "Part:BBa K2797002"

(→‎Part Characterisation)
Line 12: Line 12:
 
[[File:T--NEWCASTLE--STREPTOMYCIN ECOLI.jpeg|500px|]]
 
[[File:T--NEWCASTLE--STREPTOMYCIN ECOLI.jpeg|500px|]]
  
−
[[File:T--NEWCASTLE--SMRCHARACTERISATION.png|500px|]]
+
<img src="https://static.igem.org/mediawiki/parts/d/d5/T--NEWCASTLE--SMRCHARACTERISATION.png">
−
 
+
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 12:22, 2 October 2018


Composite part for streptomycin resistance

This composite part confers resistance to streptomycin. The part contains the constitutive BBa_J23119 promoter, the strongest promoter of the J23100 through to J23119 promoter family

Part Characterisation

T--NEWCASTLE--STREPTOMYCIN ECOLISMR.jpeg

Figure 1 E. coli strain DH5α transformed with streptomycin resistance part BBa_K2797002 growing on LB agar containing streptomycin at a concentration of 50 μg/ml

T--NEWCASTLE--STREPTOMYCIN ECOLI.jpeg 

<img src="T--NEWCASTLE--SMRCHARACTERISATION.png">

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 861
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 709
  • 1000
    COMPATIBLE WITH RFC[1000]