Difference between revisions of "Part:BBa K2797002"

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===Part Characterisation===
 
===Part Characterisation===
 
[[File:T--NEWCASTLE--STREPTOMYCIN ECOLISMR.jpeg|500px|]]
 
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'''CFPS Expression of ''J23100-deGFP'' and ''J23100-sfGFP'' Constructs:''' Time course for increase in fluorescence intensity of CFPS systems expressing GFP constructs over time. Each data point is an average of triplicate results, and error bars show +/- standard error. CFPS system with no DNA (Red) was used as the negative control.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 12:02, 2 October 2018


Composite part for streptomycin resistance

This composite part confers resistance to streptomycin. The part contains the constitutive BBa_J23119 promoter, the strongest promoter of the J23100 through to J23119 promoter family

Part Characterisation

T--NEWCASTLE--STREPTOMYCIN ECOLISMR.jpeg

CFPS Expression of J23100-deGFP and J23100-sfGFP Constructs: Time course for increase in fluorescence intensity of CFPS systems expressing GFP constructs over time. Each data point is an average of triplicate results, and error bars show +/- standard error. CFPS system with no DNA (Red) was used as the negative control.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 861
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 709
  • 1000
    COMPATIBLE WITH RFC[1000]