Difference between revisions of "Part:BBa K2558001"
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<partinfo>BBa_K2558001 short</partinfo> | <partinfo>BBa_K2558001 short</partinfo> | ||
| − | This part is derived from lux pR. They are similar except for one point mutations in the luxR binding site (G19T) and another three in the -10 region of the promotor (TAG44CTT). The functions remains the same, the mutant luxpR promotor is inducible by LuxR-AHL complex. However they do differ in gene expression strength, leakage and the expression perimeters. | + | This part is derived from lux pR https://parts.igem.org/Part:BBa_R0062. They are similar except for one point mutations in the luxR binding site (G19T) and another three in the -10 region of the promotor (TAG44CTT). The functions remains the same, the mutant luxpR promotor is inducible by LuxR-AHL complex. However they do differ in gene expression strength, leakage and the expression perimeters. |
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Revision as of 07:29, 2 October 2018
lux pR-HS
This part is derived from lux pR https://parts.igem.org/Part:BBa_R0062. They are similar except for one point mutations in the luxR binding site (G19T) and another three in the -10 region of the promotor (TAG44CTT). The functions remains the same, the mutant luxpR promotor is inducible by LuxR-AHL complex. However they do differ in gene expression strength, leakage and the expression perimeters.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]