Difference between revisions of "Part:BBa K2609002:Design"

(Design Notes)
(Source)
Line 15: Line 15:
 
===Source===
 
===Source===
  
T4 bacteriophage genome, sequence from GenBank (https://www.ncbi.nlm.nih.gov/nuccore/HM137666).
+
T4 bacteriophage genome, sequence from GenBank: https://www.ncbi.nlm.nih.gov/nuccore/HM137666
  
 
===References===
 
===References===

Revision as of 05:27, 2 October 2018


T4 bacteriophage long tail fiber protein gp37 (Modification I)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 424
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was codon optimized for expression in E. coli. The following changes were made to the wild-type protein sequence. ____

A double stop codon is placed at the end to ensure proper termination.

Source

T4 bacteriophage genome, sequence from GenBank: https://www.ncbi.nlm.nih.gov/nuccore/HM137666

References