Difference between revisions of "Part:BBa J70004"
 
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<partinfo>BBa_J70004 short</partinfo>
 
<partinfo>BBa_J70004 short</partinfo>
  
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Cloning plasmid used by BioBasic for iGEM parts. This plasmid does not have a standard BioBrick cloning site.
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Cloning plasmid used by BioBasic for iGEM parts. This plasmid does not have a standard BioBrick cloning site.  It is '''Ampicillin''' resistant.
  
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Will be adding information once obtained from Justin Pahara (iGEM 07, Alberta).  Vector is AmpR.
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Additional Information (from BioBasic): 
 +
* CAP protein binding site - 615-578 (compl. strand); 
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* mRNA (LacZ) starts at nt position 531 (compl. strand);  
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* lac repressor binding site -531-511 (compl. strand). 
 +
 
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Plasmid pUC57, 2710 bp in length, is a derivative of pUC19. pUC57 MCS contains 6
 +
restriction sites with protruding 3'-ends, that are resistant to E.coli exonuclease III. This
 +
vector is designed for cloning and generation of ExoIII deletions. The exact position of
 +
genetic elements is shown on the map (termination codons included). DNA replication
 +
initiates at position 890 (+/- 1) and proceeds in indicated direction. The bla gene
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nucleotides 2510-2442 (compl. strand) code for a single peptide.
 +
 
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The map (below) shows enzymes that cut pUC57 DNA once. Enzymes produced by Fermentas
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are shown in blue. The coordinates refer to the position of the first nucleotide in each
 +
recognition sequence.
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[[Image:PUC57_map.jpg|300px]]
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 17:41, 24 January 2008

BioBasic Cloning Plasmid pUC57

Cloning plasmid used by BioBasic for iGEM parts. This plasmid does not have a standard BioBrick cloning site. It is Ampicillin resistant.

Additional Information (from BioBasic):

  • CAP protein binding site - 615-578 (compl. strand);
  • mRNA (LacZ) starts at nt position 531 (compl. strand);
  • lac repressor binding site -531-511 (compl. strand).

Plasmid pUC57, 2710 bp in length, is a derivative of pUC19. pUC57 MCS contains 6 restriction sites with protruding 3'-ends, that are resistant to E.coli exonuclease III. This vector is designed for cloning and generation of ExoIII deletions. The exact position of genetic elements is shown on the map (termination codons included). DNA replication initiates at position 890 (+/- 1) and proceeds in indicated direction. The bla gene nucleotides 2510-2442 (compl. strand) code for a single peptide.

The map (below) shows enzymes that cut pUC57 DNA once. Enzymes produced by Fermentas are shown in blue. The coordinates refer to the position of the first nucleotide in each recognition sequence.

PUC57 map.jpg

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 396
    Illegal XbaI site found at 425
    Illegal PstI site found at 453
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 396
    Illegal PstI site found at 453
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 396
    Illegal BamHI site found at 435
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 396
    Illegal XbaI site found at 425
    Illegal PstI site found at 453
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 396
    Illegal XbaI site found at 425
    Illegal PstI site found at 453
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1790
    Illegal SapI site found at 707