Difference between revisions of "Part:BBa J61007"

Latest revision as of 16:52, 21 January 2008

pKQ-upp

This vector is for constitutive expression of the E. coli upp gene conferring 5-fluorouracil sensitivity under a Pgln promoter. It is not Biobricks!

Upp mutant strains like GHFu10E05 are resistant to around 10ug/mL 5-Fu on casamino acid agar plates or minimal media. All bacteria are resistant to 5-Fu on LB plates. pKQ-upp restores the sensitivity phenotype to mutant strains and is useful as a positive control during the development of negative selections based on upp.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 657
Illegal PstI site found at 477
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 657
Illegal PstI site found at 477
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 657
Illegal BamHI site found at 2809
Illegal XhoI site found at 1925
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 657
Illegal PstI site found at 477
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 657
Illegal PstI site found at 477
Illegal AgeI site found at 430
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 750
Illegal BsaI site found at 928

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_J61007 short</partinfo>
-[[Image:JCA_J61007map.jpg]]
-This vector constitutively expresses the E. coli upp gene conferring 5-fluorouracil sensitivity.
+[[Image:JCA_J61007map.jpg]]<br>
+This vector is for constitutive expression of the ''E. coli upp'' gene conferring 5-fluorouracil sensitivity under a Pgln promoter.  '''It is not Biobricks!'''
+Upp mutant strains like GHFu10E05 are resistant to around 10ug/mL 5-Fu on casamino acid agar plates or minimal media.  All bacteria are resistant to 5-Fu on LB plates.  pKQ-upp restores the sensitivity phenotype to mutant strains and is useful as a positive control during the development of negative selections based on upp.
 <!-- Add more about the biology of this part here