Difference between revisions of "Part:BBa K2560259:Design"

 
(→‎Design Notes)
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
OOOOO
+
The part contains the whole coding region from the <i>mcr</i> gene of <i>S. tokodaii</i> (OOOOO) without the startcodon. This region is flanked by overhangs which are Phytobrick- and MoClo-compatible and by two BsaI recognition sites (Weber et al., 2011).
  
 +
GGTCTCG<b>GATG</b>-coding_region-<b>GCTT</b>TGAGACC
  
 +
 +
The sequence was codonoptimized for <i>V. natriegens</i> ATCC 14048.
 +
 +
 +
The encoded enzyme catalyzes to conversion of malonyl-CoA into 3-oxopropanoate. By combining this part with [https://parts.igem.org/wiki/index.php?title=Part:BBa_K2560262 BBa_K2560262], it is possible to produce 3-hydroxypropionate from malonyl-CoA.
  
 
===Source===
 
===Source===

Revision as of 15:43, 30 September 2018


mcr gene for Malonyl-CoA Reductase from Sulfolobus tokodaii


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 626
    Illegal BglII site found at 819
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part contains the whole coding region from the mcr gene of S. tokodaii (OOOOO) without the startcodon. This region is flanked by overhangs which are Phytobrick- and MoClo-compatible and by two BsaI recognition sites (Weber et al., 2011).

GGTCTCGGATG-coding_region-GCTTTGAGACC


The sequence was codonoptimized for V. natriegens ATCC 14048.


The encoded enzyme catalyzes to conversion of malonyl-CoA into 3-oxopropanoate. By combining this part with BBa_K2560262, it is possible to produce 3-hydroxypropionate from malonyl-CoA.

Source

OOOOO

References