Difference between revisions of "Part:BBa K2560260"

Revision as of 12:18, 30 September 2018

mcr gene for Malonyl-CoA Reductase from Chloroflexus aurantiacus

mcr encoded the enzyme Malony-CoA Reductase from C. aurantiacus. This enzyme is involved in the 3-hydroxypropionate cycle for CO2 fixation and converts malonyl-CoA into 3-hydroxypropionic acid (Strauss & Fuchs, 1993). In a two-step reaction malonyl-CoA is first reduced to 3-oxopropanoic acid and then further reduced to 3-hydroxypropionic acid.

For this part, mcr was codonoptimized for V. natriegens and then synthetisized and integrated into the vector BBa_K2560002 via BsmBI.

It was shown that a splitted version of Mcr with separated C- and N-terminal domains increases enzyme activity (Liu et al., 2013). If these splitted genes shall be used take BBa_K2560261 and BBa_K2560262.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1932
Illegal NheI site found at 3334
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 885
Illegal BamHI site found at 213
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1188
Illegal NgoMIV site found at 2005
Illegal NgoMIV site found at 3619
Illegal AgeI site found at 1270
Illegal AgeI site found at 3168
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 <i>mcr</i> encoded the enzyme Malony-CoA Reductase from <i>C. aurantiacus</i>. This enzyme is involved in the 3-hydroxypropionate cycle for CO2 fixation and converts malonyl-CoA into 3-hydroxypropionic acid (Strauss & Fuchs, 1993). In a two-step reaction malonyl-CoA is first reduced to 3-oxopropanoic acid and then further reduced to 3-hydroxypropionic acid.
-[[File:BBa K2560260 Mcr pathway.png|200px|thumb]]
+[[File:BBa K2560260 Mcr pathway.png|500px| center]]
+For this part, <i>mcr</i> was codonoptimized for <i>V. natriegens</i> and then synthetisized and integrated into the vector [https://parts.igem.org/Part:BBa_K2560002 BBa_K2560002] via BsmBI.
+It was shown that a splitted version of Mcr with separated C- and N-terminal domains increases enzyme activity (Liu et al., 2013). If these splitted genes shall be used take [https://parts.igem.org/wiki/index.php?title=Part:BBa_K2560261 BBa_K2560261] and [https://parts.igem.org/wiki/index.php?title=Part:BBa_K2560262 BBa_K2560262].