Difference between revisions of "Part:BBa K2669000:Experience"
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After transforming our cells with a low copy amplicilin plasmid containing this composite part, cell lysis and affinity chromotography were used to extract UnaG from our cells. Please note: The exact procedure can be found <a href="http://2018.igem.org/wiki/index.php?title=Team:Uppsala/UnaG"><b>here</b></a> Bilirubin tests (addition of a small amount of bilirubin to samples) allowed us to see if the UnaG was present in our samples, since as mentioned earlier UnaG fluoresces in the presence of bilirubin. </p> | After transforming our cells with a low copy amplicilin plasmid containing this composite part, cell lysis and affinity chromotography were used to extract UnaG from our cells. Please note: The exact procedure can be found <a href="http://2018.igem.org/wiki/index.php?title=Team:Uppsala/UnaG"><b>here</b></a> Bilirubin tests (addition of a small amount of bilirubin to samples) allowed us to see if the UnaG was present in our samples, since as mentioned earlier UnaG fluoresces in the presence of bilirubin. </p> | ||
| − | "https://static.igem.org/mediawiki/2018/2/20/T--Uppsala--UnaG_Comparison.png" | + | <img class="content-card-img" src="https://static.igem.org/mediawiki/2018/2/20/T--Uppsala--UnaG_Comparison.png" alt="UnaG Comparison"> |
<p><b>Figure 2:</b> Bilirubin test before/after affinity chromatography. Going from right to left the samples are:</p> | <p><b>Figure 2:</b> Bilirubin test before/after affinity chromatography. Going from right to left the samples are:</p> | ||
Revision as of 11:40, 30 September 2018
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This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
===Applications of BBa_K2669000===
After transforming our cells with a low copy amplicilin plasmid containing this composite part, cell lysis and affinity chromotography were used to extract UnaG from our cells. Please note: The exact procedure can be found here Bilirubin tests (addition of a small amount of bilirubin to samples) allowed us to see if the UnaG was present in our samples, since as mentioned earlier UnaG fluoresces in the presence of bilirubin.
Figure 2: Bilirubin test before/after affinity chromatography. Going from right to left the samples are:
- Lysed sample of the “bad” part before AC
- Lysed sample of the “good” part before AC
- "Bad" part after AC
- "Good" part after AC