Difference between revisions of "Part:BBa K2765020"

 
 
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<partinfo>BBa_K2765020 short</partinfo>
 
<partinfo>BBa_K2765020 short</partinfo>
  
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overexpress ndi1 through ligase it to pESC
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In order to overexpress the target gene ndi1, we chose Saccharomyces cerevisiae strain CEN.PK2-1C as the host and pESC-Leu as the shuttle vector. We selected two restriction sites, Xho1 and SalI, for constructing gene ndi1.
  
  

Latest revision as of 09:23, 30 September 2018


ndi1

In order to overexpress the target gene ndi1, we chose Saccharomyces cerevisiae strain CEN.PK2-1C as the host and pESC-Leu as the shuttle vector. We selected two restriction sites, Xho1 and SalI, for constructing gene ndi1.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1114
    Illegal SpeI site found at 1032
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1114
    Illegal SpeI site found at 1032
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1114
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1114
    Illegal SpeI site found at 1032
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1114
    Illegal SpeI site found at 1032
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 388