Difference between revisions of "Part:BBa K2865002"

Revision as of 15:14, 29 September 2018

[AAV9]-Left-ITR

The Inverted Terminal Repeats (ITRs) are two 145-base sequences that locate on both sides of recombinant AAV (rAAV) shuttle plasmids. They are named so because of their symmetry, which has been shown to be required for efficient multiplication of the AAV. We extracted both left and right ITRs through PCR and ligated them to each side of AAV-9 shuttle plasmid respectively. AAV-9 virus particles were then packaged and applied in therapeutic use. This part (BBa_K2865002) encodes for the left ITR of AAV-9.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2865002 short</partinfo>
-The Inverted Terminal Repeats (ITRs) are two 145-base sequences that locate on both sides of recombinant AAV (rAAV) shuttle plasmids. They are named so because of their symmetry, which was shown to be required for efficient multiplication of the AAV. We extracted both left and right ITRs through PCR and ligated them to each side of AAV-9 shuttle plasmid respectively. AAV-9 virus particles were then packaged and applied in therapeutic use. This part (BBa_K2865002) encodes for the left ITR of AAV-9.
+The Inverted Terminal Repeats (ITRs) are two 145-base sequences that locate on both sides of recombinant AAV (rAAV) shuttle plasmids. They are named so because of their symmetry, which has been shown to be required for efficient multiplication of the AAV. We extracted both left and right ITRs through PCR and ligated them to each side of AAV-9 shuttle plasmid respectively. AAV-9 virus particles were then packaged and applied in therapeutic use. This part (BBa_K2865002) encodes for the left ITR of AAV-9.
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