Difference between revisions of "Part:BBa K2623015"
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TDP circuit is used to express exocrine protein SAHS. | TDP circuit is used to express exocrine protein SAHS. | ||
In order to facilitate the characterization and purification of SAHS protein, we added the promoter J23100+RBS+SAHS protein+RBS+E1010+B0015 to pSB1C3. | In order to facilitate the characterization and purification of SAHS protein, we added the promoter J23100+RBS+SAHS protein+RBS+E1010+B0015 to pSB1C3. | ||
| − | TDP circuit is used to express exocrine protein SAHS BBa_K2623007. | + | TDP circuit is used to express exocrine protein SAHS <partinfo>BBa_K2623007<partinfo>. |
We added RFP BBa_E1010 as a reporter gene to the gene circle for us to screen and validate. Because our aim is to obtain a large number of SAHS proteins, the constant promoter J23100 is chosen to allow SAHS to be expressed continuously. The sequence encoding for the gene is preceded by a constant promoter BBa_J23100 and an RBS BBa_B0034 , and followed by a double terminator BBa_B0015. | We added RFP BBa_E1010 as a reporter gene to the gene circle for us to screen and validate. Because our aim is to obtain a large number of SAHS proteins, the constant promoter J23100 is chosen to allow SAHS to be expressed continuously. The sequence encoding for the gene is preceded by a constant promoter BBa_J23100 and an RBS BBa_B0034 , and followed by a double terminator BBa_B0015. | ||
Revision as of 14:40, 29 September 2018
Secretory-abundant heat soluble protein "SAHS " (promoter, RBS, RFP and double terminator)
SUMMARY
TDP circuit is used to express exocrine protein SAHS. In order to facilitate the characterization and purification of SAHS protein, we added the promoter J23100+RBS+SAHS protein+RBS+E1010+B0015 to pSB1C3. TDP circuit is used to express exocrine protein SAHS No part name specified with partinfo tag.